RNA Recombination Enhances Adaptability and Is Required for Virus Spread and Virulence

מאת: Xiao Y., Rouzine I.M., Bianco S., Acevedo A., Goldstein E.F., Farkov M., Brodsky L., Andino R.
פורסם ב: Cell Host and Microbe
תיאור: Mutation and recombination are central processes driving microbial evolution. A high mutation rate fuels adaptation butalso generates deleterious mutations. Recombination between two different genomes may resolve this paradox, alleviating effects of clonal interference and purging deleterious mutations. Here we demonstrate that recombination significantly accelerates adaptation and evolution during acute virus infection. We identified a poliovirus recombination determinant within the virus polymerase, mutation of which reduces recombination rates without altering replication fidelity. By generating a panel of variants with distinct mutation rates and recombination ability, we demonstrate that recombination is essential to enrich the population in beneficial mutations and purge it from deleterious mutations. The concerted activities of mutation and recombination are key to virus spread and virulence in infected animals. These findings inform a mathematical model to demonstrate that poliovirus adapts most rapidly at an optimal mutation rate determined by the trade-off between selection and accumulation of detrimental mutations. © 2016 Elsevier Inc.
SDGs : SDG 03  |  יחידות:   | מועד: 2016 |  קישור